Chilling-induced potassium leakage of cultured citrus cells |
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Authors: | Charles F. Forney Sharon J. Peterson |
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Affiliation: | USDA, ARS, 2021 South Peach Avenue, Fresno. CA93727, USA. |
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Abstract: | Callus of 'Marsh' grapefruit ( Citrus paradisi Macf.) albedo tissue was used to investigate the effect of preconditioning temperature on the rate of chilling - stimulated K+ leakage. Callus grew most rapidly at 30°C and attained a weight of about 1 g after 30 days. The rate of K.+ leakage from nonchilled callus tissue decreased as temperature decreased from 20 to 7.5°C, but no measurable change in rate was observed between 7.5 and 0°C. When calli were held for 40 days at 01 2.5 or 5°C, K+ leakage increased 200%, 60% or 0%) respectively. Holding callus for 5 days at 10 or 15°C prior to chilling for 40 days at 0°C prevented the increase in K+ leakage observed in callus receiving no preconditioning treatment. Preconditioning at 7.5 and 20°C was less effective in reducing chilling - induced leakage. Preconditioning at 10°C for 5, 2 or 1 day reduced chilling – induced leakage after 40 days at 0°C by 50%, 33% and 15%. respectively. |
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Keywords: | Callus chilling injury Citrus paradisi grapefruit preconditioning |
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