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Simple Sequence Repeat Analysis of Genetic Diversity in Primary Core Collection of Peach (Prunus persica)
引用本文:Li TH,Li YX,Li ZC,Zhang HL,Qi YW,Wang T. Simple Sequence Repeat Analysis of Genetic Diversity in Primary Core Collection of Peach (Prunus persica)[J]. 植物学报(英文版), 2008, 50(1): 102-110. DOI: 10.1111/j.1744-7909.2007.00598.x
作者姓名:Li TH  Li YX  Li ZC  Zhang HL  Qi YW  Wang T
作者单位:[1]Department of Fruit Science, College of Agronomy and Biotechnology, China Agncultural University, Beijing 100094, China [2]College of Crop Science, China Agricultural University, Beijing 100094, China [3]State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China
基金项目:Supported by Beijing Municipal Education Commission Urban Agriculture Disciplines Development Project (XK1001900553) and Program for new Century Excellent Talents in University of China (NCET-06-0108),
摘    要:In this study, the genetic diversity of 51 cultivars in the primary core collection of peach (Prunus persica (L.) Batsch) was evaluated by using simple sequence repeats (SSRs). The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data. Twenty-two polymorphic SSR primer pairs were selected, and a total of 111 alleles were identified in the 51 cultivars, with an average of 5 alleles per locus. According to traditional Chinese classification of peach cultivars, the 51 cultivars in the peach primary core collection belong to six variety groups. The SSR analysis revealed that the levels of the genetic diversity within each variety group were ranked as Sweet peach 〉 Crisp peach 〉 Flat peach 〉 Nectarine 〉 Honey Peach 〉 Yellow fleshed peach. The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars. Cluster analysis by the unweighted pair group method with arithmetic averaging (UPGMA) placed the 51 cultivars into five linkage clusters. Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster. Different variety groups could not be differentiated in accordance with SSR markers. The SSR analysis revealed rich genetic diversity in the peach primary core collection, representative of genetic resources of peach.

关 键 词:遗传多样性 桃子 生长特性 植物学
收稿时间:2006-08-01
修稿时间:2006-10-24

Simple sequence repeat analysis of genetic diversity in primary core collection of peach (Prunus persica)
Li Tian-Hong,Li Yin-Xia,Li Zi-Chao,Zhang Hong-Liang,Qi Yong-Wen,Wang Tao. Simple sequence repeat analysis of genetic diversity in primary core collection of peach (Prunus persica)[J]. Journal of integrative plant biology, 2008, 50(1): 102-110. DOI: 10.1111/j.1744-7909.2007.00598.x
Authors:Li Tian-Hong  Li Yin-Xia  Li Zi-Chao  Zhang Hong-Liang  Qi Yong-Wen  Wang Tao
Affiliation:(Department of Fruit Science, College of Agronomy and Biotechnology, China Agricultural University;, Beijing 100094, China;College of Crop Science, China Agricultural University;, Beijing 100094, China;State Key Laboratories for Agrobiotechnology, China Agricultural University;, Beijing 100094, China)
Abstract:In this study, the genetic diversity of 51 cultivars in the primary core collection of peach (Prunus persica (L.) Batsch) was evaluated by using simple sequence repeats (SSRs). The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data. Twenty-two polymorphic SSR primer pairs were selected, and a total of 111 alleles were identified in the 51 cultivars, with an average of 5 alleles per locus. According to traditional Chinese classification of peach cultivars, the 51 cultivars in the peach primary core collection belong to six variety groups. The SSR analysis revealed that the levels of the genetic diversity within each variety group were ranked as Sweet peach > Crisppeach > Flat peach > Nectarine > Honey Peach > Yellow fleshed peach. The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars. Cluster analysis by the unweighted pair group method with arithmetic averaging (UPGMA)placed the 51 cultivars into five linkage clusters. Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster. Different variety groups could not be differentiated in accordance with SSR markers. The SSR analysis revealed rich genetic diversity in the peach primary core collection, representative of genetic resources of peach.
Keywords:genetic diversity  peach (Prunus persica)  primary core collection  simple sequence repeat (SSR)  unweighted pair group method with arithmetic average (UPGMA).
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