Some effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes |
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| Authors: | Parodi Jorge Romero Fernando Miledi Ricardo Martínez-Torres Ataúlfo |
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| Affiliation: | a Laboratorio de neurociencias-CEBIOR, Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de la Frontera, Av. Francisco Salazar 01145, P.O. Box 54-D, Temuco, Chile
b Laboratorio Neurobiología Molecular y Celular II, Departamento de Neurobiología Molecular y Celular, Instituto de Neurobiología, Campus Juriquilla-Querétaro, UNAM, Mexico |
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| Abstract: | A study was made of the effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes. 1 μg/ml of the venom made the resting plasma membrane potential more negative in cells voltage-clamped at −60 mV. The effect was potentially due to the closure of one or several conductances that were investigated further. Thus, we determined the effects of the venom on the following endogenous ionic-currents: (a) voltage-activated potassium currents, (b) voltage-activated chloride-currents, and (c) calcium-dependent chloride-currents (Tout). The results suggest that the venom exerts its action mainly on a transient outward potassium-current that is probably mediated by a Kv channel homologous to shaker. Consistent with the electrophysiological evidence we detected the expression of the mRNA coding for xKv1.1 in the oocytes. |
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| Keywords: | Venom Latrodectus mactans Electrophysiology Oocytes Potassium channels Xenopus laevis |
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