Fatty acids differentially modify the expression of urokinase type plasminogen activator receptor in monocytes |
| |
Authors: | Assmann Anke Möhlig Matthias Osterhoff Martin Pfeiffer Andreas F H Spranger Joachim |
| |
Institution: | a Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA b Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Department of Endocrinology, Diabetes and Nutrition, Hindenburgdamm 30, 12200 Berlin, Germany c German Institute of Human Nutrition Potsdam, Department of Clinical Nutrition, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany |
| |
Abstract: | The urokinase plasminogen activator system with its receptor uPAR contributes to the migratory potential of macrophages, a key event in atherosclerosis. We here investigated whether free fatty acids (FFA) modify the expression for uPAR in the PMA-differentiated human monocyte/macrophage-like cell line U937. Two hundred micromolar palmitate induced a threefold increase of the uPAR mRNA expression. Although the mono- and polyunsaturated fatty acids oleate and linoleate also stimulated uPAR expression, oleate had a significantly lower effect than palmitate. The observed effects were time and dose dependent. Inhibition of PKC-and ERK-pathways resulted in a strong down-regulation of basal uPAR expression whereas the FFA induced up-regulation remained unchanged. In contrast, FFA induced uPAR up-regulation was abolished by the specific inhibition of p38 MAPK. In conclusion we demonstrate that uPAR expression in human monocytes/macrophages is differentially stimulated by FFA. These effects are partially mediated by the p38 MAP-kinase signaling pathway. |
| |
Keywords: | Urokinase type plasminogen activator receptor (uPAR) Free fatty acids Monocytes/macrophages P38 MAP-kinase Atherosclerosis |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|