Use of two-dimensional thin-layer chromatography for the components study of poly(adenosine diphosphate ribose) |
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| Authors: | G Keith J Desgrès G de Murcia |
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| Affiliation: | IBMC du CNRS et de l'Université Louis Pasteur, Strasbourg, France. |
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| Abstract: | Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly(ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly(ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length. (ii) The branching points are located at regular distances of approximately 41 derivatives from each other. |
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