A computer program to calculate and design oligonucleotide primers from amino acid sequences |
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| Authors: | Nash John H.E. |
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| Affiliation: | Institute for Biological Sciences, National Research Council of Canada Ottawa, Ontario, Canada K1A 0R6 |
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| Abstract: | A computer program to generate oligonucleotide sequences frompeptide data is presented. The program, PrimerGen, reverse-translatesa peptide string into its corresponding degenerate deoxyribonucleotidecounterpart, and generates sequences of oligonucleotide moleculeswhich can be synthesized and either used as probes for hybridizationsor as primers in polymerase chain reactions. |
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