Membrane fluidity in glutamic acid-producing bacteria Brevibacterium sp. ATCC 13869 |
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Authors: | Michael Neubeck Elmar Prenner Predrag Horvat Rudolfo Bona Albin Hermetter Anton Moser |
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Institution: | (1) Institut für Biotechnologie, Technische Universität Graz, Petersgasse 12/1, A-8010 Graz, Austria;(2) Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Petersgasse 12/2, A-8010 Graz, Austria;(3) Present address: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 41000 Zagreb, Croatia |
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Abstract: | The bacterial secretion of glutamate was studied through plasma membrane fluidity, measured by anisotropy using the fluorophore TMA-DPH incorporated in the lipid part of the cell membrane. Cells of Brevibacterium sp. ATCC 13869 (wild type) were switched from the biotin-limited, producing state to the biotin-supplemented, non-producing state, and back. The following conclusions could be drawn: 1. It was not possible to detect any change in anisotropy by switching the cells from biotin-limited biotin-supplemented, as well as from biotin-supplemented, to biotin-limited, media. 2. The anisotropy value in the glutamic acid fermentation remains constant during the lag, exponential, growth, production and stationary phases. 3. The treatment of cells with a neutral synthetic polyester of ethylene-and propyleneoxide with soya oil-fatty acids increased the anisotropy values, indicating incorporation of the surfactant. 4. Glutamate secretion is not coupled with membrane fluidity, so a leak providing a general fluidization of the membrane could not be detected. |
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Keywords: | Anisotropy Glutamic acid Membrane fluidity Brevibacterium |
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