| 电子顺磁共振测量蛋白质分子内选定位点之间距离的方法及应用 |
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| 引用本文： | 吴可. 电子顺磁共振测量蛋白质分子内选定位点之间距离的方法及应用[J]. 生物物理学报, 2010, 0(4) |
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| 作者姓名： | 吴可 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所; |
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| 基金项目： | 国家自然科学基金项目(30970693,30750009); 国家科技部创新方法专项课题(2008IM022000)~~ |
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| 摘 要： | 蛋白质分子的结构决定了其特性和功能,准确测量蛋白质分子中特殊位点之间的距离对其结构解析至关重要。该文在简要介绍电子顺磁共振(electron paramagnetic resonance,EPR)方法测量蛋白质分子内未偶自旋之间距离基本原理的基础上,重点综述了近年来EPR结合定点自旋标记(site-directed spin label,SDSL)技术在研究蛋白质结构与功能方面的应用情况,归纳了EPR-SDSL方法测量距离的特点和存在问题,并提出了改进用连续波EPR技术测量距离的准确度的思路和实现方法。
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| 关 键 词： | 电子顺磁共振 定点自旋标记 距离测量 蛋白质结构 |
Technique and Application of Distance Measurement between Selected Points in Protein Molecule by EPR |
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| WU Ke Beijing Institute of Radiation Medicine,The Academy of Military Medical Sciences,Beijing ,China. Technique and Application of Distance Measurement between Selected Points in Protein Molecule by EPR[J]. Acta Biophysica Sinica, 2010, 0(4) |
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| Authors: | WU Ke Beijing Institute of Radiation Medicine The Academy of Military Medical Sciences Beijing China |
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| Affiliation: | WU Ke Beijing Institute of Radiation Medicine,The Academy of Military Medical Sciences,Beijing 100850,China |
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| Abstract: | The properties and biological functions of protein depend on its structure. Structure determination is strongly dependent on accurate measurement of distance between selected sites in protein. The principle distance measurement by EPR between uncoupled spins was introduced. Furthermore, progress in site-directed spin labeling (SDSL) EPR technique applied in protein structure and function research, was reviewed. |
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| Keywords: | EPR SDSL Distance measurement Protein structure |
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