Labeling of proteins and oligopeptides with luminescent lanthanide(III) chelates. |
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Authors: | Lassi Jaakkola Jari Peuralahti Harri Hakala Veli-Matti Mukkala Pertti Hurskainen Jari Hovinen |
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Institution: | PerkinElmer Life and Analytical Sciences, Turku Site, P.O. Box 10, FIN-20101 Turku, Finland. |
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Abstract: | Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine-based europium(III) chelate designed for solution phase protein labeling is demonstrated. |
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Keywords: | lanthanide(III) chelates labeling solid phase solution phase |
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