Theoretical basis for reducing time-lines to the determination of positive Mycobacterium tuberculosis cultures using thymidylate kinase (TMK) assays |
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Authors: | Misaki Wayengera |
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Institution: | (1) Division of Molecular Pathology, Dept of Pathology, School of Biomedical Sciences, College of Health Sciences, Makerere University, PO Box 7072, Kampala, Uganda;(2) Restrizymes Biotherapeutics Uganda Limited, PO Box 16606, Kampala, Uganda;(3) School of Health Sciences, Kampala International University Western Campus, PO Box 71, Ishaka, Uganda |
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Abstract: | Background
In vitro culture of pathogens on growth media forms a "pillar" for both infectious disease diagnosis and drug sensitivity profiling.
Conventional cultures of Mycobacterium tuberculosis (M.tb) on Lowenstein Jensen (LJ) medium, however, take over two months to yield observable growth, thereby delaying diagnosis and
appropriate intervention. Since DNA duplication during interphase precedes microbial division, "para-DNA synthesis assays"
could be used to predict impending microbial growth. Mycobacterial thymidylate kinase (TMKmyc) is a phosphotransferase critical
for the synthesis of the thymidine triphosphate precursor necessary for M.tb DNA synthesis. Assays based on high-affinity detection of secretory TMKmyc levels in culture using specific antibodies are
considered. The aim of this study was to define algorithms for predicting positive TB cultures using antibody-based assays
of TMKmyc levels in vitro. |
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Keywords: | |
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