31P-NMR spectra of the Ha-ras p21.nucleotide complexes |
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| Authors: | P R?sch A Wittinghofer J Tucker G Sczakiel R Leberman I Schlichting |
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| Affiliation: | 1. Institute of Coal Chemistry, Chinese Academy of Sciences, 27 South Taoyuan Road, Taiyuan 030001, People''s Republic of China;2. College of Science, Shanghai University, Shanghai 200444, People''s Republic of China;3. Taiyuan Supervision and Testing Station for Quality of Grain, Taiyuan 030013, People''s Republic of China;4. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, People''s Republic of China;5. Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark |
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| Abstract: | Phosphorus nuclear magnetic resonance spectra of the Ha-ras oncogene product p21 and its nucleotide complexes have been obtained. It is shown that the 31P nuclear magnetic resonance spectra of a number of nucleotide-enzyme complexes show some common features. In particular, the chemical shift values of the beta-phosphorus resonance of enzyme-bound NTP and NDP (N = A, G) of hydrolases exhibit a downfield shift virtually identical for myosin, elongation factor Tu, and the Ha-ras oncogene product p21. This suggests that the stereochemistry around the beta-phosphorus might be similar in these compounds. |
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