Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid |
| |
Authors: | Yoshiya Seto Hideyuki Matsuura Mana Matsushige Kosaku Takahashi Hiroyuki Ito Kensuke Nabeta |
| |
Institution: | a Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku Sapporo 060-8589, Japan b Laboratory of Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo 060-8589, Japan c Laboratory of Cell Biology and Manipulation, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo 060-8589, Japan |
| |
Abstract: | Tuberonic acid (12-hydroxy epi-jasmonic acid, TA) and its glucoside (TAG) were isolated from potato leaflets (Solanumtuberosum L.) and shown to have tuber-inducing properties. The metabolism of jasmonic acid (JA) to TAG in plant leaflets, and translocation of the resulting TAG to the distal parts, was demonstrated in a previous study. It is thought that TAG generated from JA transmits a signal from the damaged parts to the undamaged parts by this mechanism. In this report, the metabolism of TA in higher plants was demonstrated using 12-3H]TA, and a glucosyltransferase active toward TA was purified from the rice cell cultures. The purified protein was shown to be a putative salicylic acid (SA) glucosyltransferase (OsSGT) by MALDI-TOF-MS analysis. Recombinant OsSGT obtained by overexpression in Escherichia coli was active not only toward TA but also toward SA. The OsSGT characterized in this research was not specific, but this is the first report of a glucosyltransferase active toward TA. mRNA expressional analysis of OsSGT and quantification of TA, TAG, SA and SAG after mechanical wounding indicated that OsSGT is involved in the wounding response. These results demonstrated a crucial role for TAG not only in potato tuber formation, but also in the stress response in plants and that the SA glucosyltransferase can work for TA glucosylation. |
| |
Keywords: | Oryza sativa Gramineae Glycine max Leguminosae Solanum tuberosum Solanaceae Solanum lycopersicum Solanaceae Nicotiana tabacum Solanaceae Zea mays Gramineae Arabidopsis thaliana Cruciferae Albizzia julibrissin Leguminosae Salvia officinalis Lamidaceae Tuberonic acid Tuberonic acid glucoside Tuberonic acid glucosyltransferase Jasmonic acid Wounding stress Salicylic acid glucosyltransferase |
本文献已被 ScienceDirect 等数据库收录! |
|