Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells |
| |
Authors: | Natali R Mustafa Young Hae Choi Alfons WM Lefeber Robert van der Heijden |
| |
Institution: | a Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Einsteinweg 55, Leiden, The Netherlands b Division of NMR, Institute of Chemistry, Leiden University, Leiden, The Netherlands c Division of Analytical Biosciences, Leiden-Amsterdam Center of Drug Research, Leiden University, Leiden, The Netherlands |
| |
Abstract: | Feeding experiments using 1-13C]-d-glucose to Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by 13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space. |
| |
Keywords: | Salicylic acid biosynthesis 2 3-Dihydroxybenzoic acid Isochorismate pathway Phenylalanine pathway Catharanthus roseus cell suspension cultures |
本文献已被 ScienceDirect 等数据库收录! |
|