Institution: | a Université d’Orléans, UFR-Faculté des Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, rue de Chartres, BP 6759, F-45067 Orléans Cedex 02, France b INRA, USC2030 ‘Arbres et Réponses aux Contraintes Hydrique et Environnementales’ (ARCHE), F-45067 Orléans Cedex 02, France c INRA, Nancy Université, UMR1137 INRA-UHP ‘Ecologie et Ecophysiologie Forestières’, IFR 110 ‘Génomique, Ecophysiologie et Ecologie Fonctionnelle’, INRA Nancy, F-54280 Champenoux, France d INRA, UMR1202 BIOGECO, Equipe de Génétique, 69 route d’Arcachon, F-33612 Cestas, France e Pôle Protéomique de la Plateforme Génomique Fonctionnelle Bordeaux, Université de Bordeaux 2, F-33076 Bordeaux, France f INRA, Plate-forme de Protéomique Analytique et Fonctionnelle, Laboratoire de Spectrométrie de Masse, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France g CNRS, UMR6175, F-37380 Nouzilly, France h Université de Tours, F-37041 Tours, France i Haras Nationaux, F-37380 Nouzilly, France |
Abstract: | Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides × Populus nigra, cv. ‘Agathe_F’ and ‘Cima’, were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra.A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, ‘Cima’ displayed the highest abundance of antioxidant enzymes.In response to water deficit, about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while ‘Cima’ differed from ‘Agathe_F’ by increased abundance of enzymes involved in photorespiration and in oxidative stress, ‘Agathe_F’ was mainly differentiated by increased abundance of enzymes involved in photosynthesis. |