| 水稻叶绿体DNA克隆片段的分析和rbcL基因在重组质粒上的定位 |
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| 引用本文： | 沈志伟,孙崇荣,曹凯鸣,詹树萱.水稻叶绿体DNA克隆片段的分析和rbcL基因在重组质粒上的定位[J].遗传学报,1989(6). |
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| 作者姓名： | 沈志伟 孙崇荣 曹凯鸣 詹树萱 |
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| 作者单位： | 复旦大学生物化学系 上海
(沈志伟，孙崇荣，曹凯鸣)，复旦大学生物化学系 上海(詹树萱) |
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| 基金项目： | 国家“75”科研基金，复旦大学开放实验室基金 |
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| 摘 要： | 杂交灿稻(珍汕97B)的叶绿体DNA克隆到pBR 322载体上后,从克隆库中筛选出含核酮糖1.5-二磷酸羧化酶/加氧酶大亚基基因(rbcL)的重组子(19.3kb),用10种限制性内切酶分析了这个重组质粒并制作了完整的物理图谱,rbcL基因被定位在这个物理图谱上。
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| 关 键 词： | 水稻叶绿体 基因克隆 核酮糖1.5-二磷酸羧化酶/加氧酶基因 物理图谱 |
Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid |
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| Shen Zhiwei Sun Chongrong Cao Kaiming Zhan Shuxuan.Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid[J].Journal of Genetics and Genomics,1989(6). |
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| Authors: | Shen Zhiwei Sun Chongrong Cao Kaiming Zhan Shuxuan |
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| Abstract: | The pure rice (Oryza sativa,subsp.Indica,cv.Zhenshan 97B) chloroplast DNA was dig-ested by restriction enzyme BamHl and the resulting fragments were ligated to the BamHI site of pBR322.One recombinant plasmid which contains a 19.3kb insertel DNA fragment was isolated from the clone bank and was named pOSBl.A physical map of the recombinant plasmid was constructed by cleavage with ten restriction endonucleases,and the gene rbcL was located on die pOSB1 |
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| Keywords: | Rice chloroplast DNA Gene cloning rbcL Physical map |
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