pHG276: A multiple cloning site pBR322 copy number vector expressing a functional lacα peptide from the bacteriophage lambda PR promoter |
| |
Authors: | Gordon S. A. B. Stewart Sharon Lubinsky-Mink Jonathan Kuhn |
| |
Abstract: | The bacteriophage lambda early promoter PR has been used to direct the synthesis of lacα in a plasmid containing the multiple cloning site of pUC8. The copy number of the plasmid is controlled by the rop(rom) gene and the plasmid incorporates the cI857 gene for temperature regulation of lacα expression. Isolation of recombinant derivatives with DNA inserts in the multiple cloning region can be identified by insertional inactivation of lacα and consequently, a Lac− phenotype in a host carrying the M15 deletion of lac. The potential of pHG276 as a fully regulated expression vector is examined. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |