Liposome filtration. Dependence on transition temperature |
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Authors: | Avrom M Brendzel Irving F Miller |
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Institution: | Bioengineering Program, University of Illinois at Chicago Circle, Chicago, IL 60680 U.S.A. |
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Abstract: | Liposomes formed by vortexing and passed through polycarbonate surface retention membranes showed appreciable differences in filtration behavior depending on the temperature of filtration relative to the liposome gel-liquid crystal transition temperature. Below transition, liposomes were filterable and size distributions could be determined; the cumulative volume distributions were log-normal. Above transition, liposomes were not filterable: smaller liposomes were formed until a limiting size was reached. These results suggest that liquid crystal liposome size distributions cannot be determined by filtration. This filtration behavior is a physical property of liposomes, related to the gel-liquid crystal transition, not previously reported. This property could be exploited as a new method for controlling liposome size distributions, but the implications for lipid membranes, including biological membranes, are general. |
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Keywords: | Liposome filtration Transition temperature Gel-liquid crystal |
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