Carbodiimides inhibit the acid-induced purple-to-blue transition of bacteriorhodopsin |
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| Authors: | Robert Renthal Bill Wallace |
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| Affiliation: | 1. Division of Earth and Physical Sciences, University of Texas at San Antonio, San Antonio, TX 78285 U.S.A.;2. Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78284 U.S.A. |
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| Abstract: | Reaction of purple membrane with water soluble carbodiimides inhibits the spectral transition from purple to blue observed at acid pH. The pK and Hill constant for this transition are shifted from 3.4 to 2.6 and from 1.8 to 0.85, respectively. The results suggest a connection between the uptake side of the proton pump and the purple-to-blue transition. |
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| Keywords: | Bacteriorhodopsin Purple membrane Membrane labeling Carbodiimide Proton pump |
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