应用频数分布面积法定位家蚕QTL

利用生态型差异较大的两个家蚕品种７８２和ｏｄ１００作正反杂交，得到Ｆ１。让Ｆ１雄蚕与叠双隐性标记的ｏｄ１００回 到回交子代。

Mapping QTLs in Silkworm by Distribution Area of Frequencies

Two silkworm varieties,782 and odl00,which are quite different form each other in ecotype,were positively and oppositely crossed to generate F1.Male silkworm mothes of Fl were crossed with female mothes of od100 with two recessive morphomarkers to generate backcross offspring.The frequent distributions of two quantitative traits,weight of cocoon shell and of cocoon,of backcross offspring in four morphomarker genotypes were made respectively and then the QTLs for these two quantitative traits were mapped by using method of distribution area of frequencies (DAF).The weight of cocoon shell and of cocoon were respectively controlled by a major gene on the sex-linkage group.A QTL for weight of cocoon shell at 58.14cM distance to flank of od gene,and a QTL for weight of cocoon is completely linked with sch gene.The linkage distance between the two morphomarkers,sch and od is 20.2cM.The fact that the various actual distribution patterns of frequencies of these two quantitative traits in backcross(testcross)offspring were identical with the expectant distribution of frequencies implicates that the DAF method is useful for mapping QTLs using two morphmarkers.