Oxalate-degrading Enterococcus faecalis |
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Authors: | Hokama S Honma Y Toma C Ogawa Y |
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Institution: | Department of Urology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan. |
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Abstract: | An oxalate-degrading Enterococcus faecalis was isolated from human stools under anaerobic conditions. The bacteria required a poor nutritional environment and repeated subculturing to maintain their oxalate-degrading ability. The E. faecalis produced 3 proteins (65, 48, and 40 kDa) that were not produced by non-oxalate-degrading E. faecalis as examined by SDS-PAGE. Antibodies against oxalyl-coenzyme A decarboxylase (65 kDa) and formyl-coenzyme A transferase (48 kDa) obtained from Oxalobacter formigenes (an oxalate-degrading anaerobic bacterium in the human intestine) reacted with 2 of the proteins (65 and 48 kDa) from the E. faecalis as examined by Western blottings. This is the first report on the isolation of oxalate-degrading facultative anaerobic bacteria from humans. |
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Keywords: | enterococcus faecalis oxalate human stool |
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