Production of cloned carboxypeptidase G2 by Escherichia coli: Genetic and environmental considerations |
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Authors: | M. J. Lancaster R. J. Sharp J. R. Court I. D. McEntee R. G. Melton R. Sherwood |
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Affiliation: | (1) Division of Biotechnology, PHLS, Centre for Applied Microbiology and Research, Porton Down, SP4 0JG Salisbury, Wilts |
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Abstract: | Summary The optimum production of cloned carboxypeptidase G2 from plasmid pNM21 byEscherichiacoli was found to be strongly strain- and temperature-dependent. The superior host was strain RV308 and the preferred growth temperature 28°C. Copy number, which decreased during exponential growth of all strains examined, did not relate in these studies to the level of enzyme production: the strain with the highest enzyme yield also having the lowest overall copy number. |
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