Long-term in vitro cultivation of Plasmodium berghei |
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| Institution: | 1. The Wistar Institute, Philadelphia, Pennsylvania, USA;2. Maternal-Infant Study Center (CEMI), University of Puerto Rico, San Juan, Puerto Rico;3. School of Biological Sciences and Nebraska Center for Virology, University of Nebraska, Lincoln, Nebraska, USA;4. Duke Human Vaccine Institute, Duke University, Durham, North Carolina, USA;5. Department of Biostatistics and Epidemiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA;6. Division of Biostatistics and Epidemiology, University of Massachusetts, Amherst, Massachusetts, USA;7. University of Pennsylvania Health System, Philadelphia, Pennsylvania, USA;8. Department of Microbiology, UPR Medical School, San Juan, Puerto Rico;1. Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751, Republic of Korea;2. Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 363-951, Republic of Korea;3. Department of Microbiology, Institute of Health Sciences and PMBBRC, Gyeongsang National University School of Medicine, Jinju 660-751, Republic of Korea;4. Department of Tropical Medicine, and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 400-712, Republic of Korea;1. Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium;2. Laboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium;3. Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health, ARC-Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa;1. Centre of Influenza Research and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China;2. State Key Laboratory of Emerging Infectious Disease, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China;3. Department of Pathology, Queen Mary Hospital, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China;4. Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Saudi Arabia;5. Department of Clinical Studies, College of Veterinary Medicine, King Faisal University, Saudi Arabia;6. Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt;7. Division of Virology, Department of Infectious Diseases, St Jude Children''s Hospital, Memphis, TN, USA;8. Center of Excellence for Influenza Viruses, National Research Center, Giza, Egypt;1. Department of Epidemiology and Biostatistics, Institute for Global Health Sciences, University of California, San Francisco, CA 94158, USA;2. Eijkman Research Center for Molecular Biology, National Research and Innovation Agency, Indonesia;3. Department of Parasitology, Faculty of Medicine, Hasanuddin University, Indonesia |
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| Abstract: | Long-term in vitro culture of Plasmodium berghei was established using the Petri dish candle jar method of Trager and Jensen (1976). Cultures were established at 22, 27 and 37°C. As optimal growth was observed at 27°C, subsequent cultivation was carried out at this temperature. RPMI 1640 medium was modified by incorporating additional glucose (1 mg ml−1) and bactopeptone (1 mg ml−1) in the medium. This medium was found suitable for maintenance of mouse erythrocytes in vitro. P. berghei cultures were maintained using candle jars and this modified RPMI 1640 medium for 45 weeks. |
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