Synthesis of adipokinetic hormone (AKH-I) in the locust brain |
| |
| Affiliation: | 1. Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76–100, Israel;2. Genentech Inc., South San Francisco, CA 94080, U.S.A.;1. Department of Animal Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa;2. Fisheries Management Branch, Department of Agriculture, Forestry and Fisheries, Roggebaai, 8012, South Africa;3. Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains 4108, Australia;1. Laboratory of Biology, Sapporo Campus, Hokkaido University of Education, Sapporo, 002-8502, Japan;2. Research Center for Structural Materials, National Institute for Materials Science, Tsukuba, 305-0044, Japan;1. College of Ocean and Earth Sciences, Xiamen University, Xiamen, China;2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;3. Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;4. National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;5. School of Life Sciences, Nantong University, Nantong 226000, China;6. College of Science & Engineering, James Cook University, Townsville, Queensland, Australia;1. Insect Ecology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Mie, Japan;2. Graduate School of Agriculture, Tamagawa University, Machida, Tokyo, Japan |
| |
| Abstract: | Methanol extracts of vitellogenic female locust brains contain two factors that inhibit protein synthesis in fat body tissue excised from such individuals. One of these factors (BI) elicits lipid mobilization when injected into adult male locusts. The retention times of BI on an RP-18 column and on an RP-4 column are identical to those of synthetic locust adipokinetic hormone (AKH-I) on each of these columns. Half maximal inhibition of protein synthesis in excised adult locust fat bodies is exerted by 0.05 brain extract equivalents of BI, which is equivalent to activity elicited by 1.5 pmol of AKH-I, as previously determined by AKH-radioimmunoassay. Enzymatic hydrolysis of the N-terminal pyroglutamate, followed by amino acid sequence analysis, indicates that the structure of BI is similar to that of the decapeptide AKH-I synthesized in the glandular lobe of the locust corpora cardiaca (CC). Incorporation of [5-3H]tryptophan into BI of locust brains incubated in vitro indicates that the AKH-I present in the brain is synthesized in situ and is not transported from the CC. Similar incorporation of radiolabel into AKH-I is obtained when excised CC are incubated in vitro. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
