Breakdown of locust adipokinetic hormone I by malpighian tubules of Schistocerca gregaria |
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| Affiliation: | 1. Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Via delle Cascine 5, 50144 Firenze, Italy;2. CNR-Istituto di Cristallografia, Via Paolo Gaifami 18, 95126 Catania, Italy;3. Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via F. Delpino 1, 80137 Napoli, Italy;4. Department of Agricultural, Forest and Food Sciences, University of Turin, L.go P. Braccini 2, 10095 Grugliasco, Italy;5. Department of Organic Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 13, 50019 Sesto Fiorentino, Firenze, Italy;1. Pierre Fabre Dermo-Cosmétique, Pierre Fabre R&D Center, Applied Research Department, Avenue Hubert Curien, Cedex 01, 31025 Toulouse, France;2. Luxembourg Institute of Science and Technology (LIST), Advanced Characterization Platform, Materials Research and Technology, 41, rue du Brill, L-4422 Belvaux, Luxembourg;3. Laboratoires Avène, Lavaur, France;4. ImaBiotech SAS, Parc Eurasanté, 885 Avenue Eugène Avinée, 59120 Loos, France |
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| Abstract: | The degradation of synthetic adipokinetic hormone I (AKHI) was studied using homogenates of the Malpighian tubules (MTs) from Schistocerca gregaria. Three major breakdown products, AKHI-1, AKHI-2 and AKHI-3, were found when the reaction mixture was subjected to reversed-phase high-performance liquid chromatography (RP-HPLC). At pH 7.5 AKHI-1 and AKHI-3 were found in small amounts only, however, at pH 8.0 large amounts could be detected. It was concluded that more than one degradative enzyme must be responsible for the complete breakdown of AKHI. Analysis of the products showed that AKHI-1 contained the AKHI residues 1–6 (pGlu-Leu-Asn-Phe-Thr-Pro), AKHI-2 only residue 8 (Trp) and AKHI-3 residues 8–10 (Trp-Gly-Thr-NH2). None of the breakdown products exhibited lipid-mobilizing activity when tested at doses of 20 pmol per locust. |
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