Soluble glutathione transferase isoenzymes in Daphnia magna straus and their interaction with 2,4-dichlorophenoxyacetic acid and 1,4-benzoquinone |
| |
| Institution: | 1. Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, PR China;2. Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian, 116023, PR China |
| |
| Abstract: | The glutathione transferase (GST) activity in the cytosol of the water flea Daphnia magna Straus was partially purified by glutathione affinity chromatography. Chromatofocusing on the Polybuffer exchangers 94 and 118 separated the GST isoenzymes in one neutral and four cationic forms, and some minor fractions one of which was an anionic form. The major GST isoenzymes were partially characterized by different biochemical parameters. The water pollutants 2,4-dichlorophenoxyacetic acid and 1,4-benzoquinone inhibited the water flea GST isoenzymes, following the same kinetic inhibition patterns as for rat liver GST. It is concluded that water flea GST can play an important role in the detoxification of aquatic pollutants. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
