Changes in calmodulin levels during development of the silkworm,Bombyx mori |
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| Affiliation: | 1. College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China;2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, China;3. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization of Sichuan Province, Southwest Minzu University, Chengdu, 610041, China;4. Key Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, 610041, China;5. Longri Breeding Stock Farm of Sichuan Province, Dujiangyan, 611800, China;1. College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, PR China;2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, 610041, PR China;3. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province, Chengdu, 610041, PR China;4. Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, 610041, PR China;5. College of Animal Sciences and Technology, Gansu Agricultural University, Lanzhou, 730070, PR China;1. Animal Technology and Research Center (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain;2. Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain |
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| Abstract: | Calmodulin levels were measured in various tissues during the larval-adult development of the silkworm, Bombyx mori. In the larval period, calmodulin levels in fat body, midgut and testis were in a range of 0.3–1.7 μg/mg protein and remained almost constant during larval growth. The silk gland contained a relatively high (0.2 μg/mg protein) level of calmodulin early in the fifth instar which gradually decreased during maturation of the larva. At pupation, testis calmodulin dropped from 1.5 to 1.7 μg/mg protein to about 1 μg/mg, and remained constant thereafter. The most striking change occurred in fat body calmodulin which fell from 0.5 to 0.6 μg/mg in the larval stage to 0.01–0.03 μg/mg during pupal-adult metamorphosis. Midgut calmodulin levels were unchanged at pupation and remained constant during pupal-adult development.When expressed on per g wet weight basis, calmodulin levels in silkworm tissues were comparable to mammalian tissue levels. However, only 2–4% of the total calmodulin in silkworm tissues was in a membrane-bound form compared to 20–60% for membrane-bound calmodulin in mammals. |
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