In vitro biosynthesis of JH III by the corpora allata of adult females of Blattella germanica (L) |
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| Institution: | 1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People''s Republic of China;2. School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen, 518060, People''s Republic of China;3. School of Pharmacy, Jinan University, Guangzhou, 510632, People''s Republic of China;4. Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, People''s Republic of China;1. Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand;2. Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand;3. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand;4. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;5. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand;1. State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Taicheng Road, Yangling, Shaanxi 712100, China;2. Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA |
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| Abstract: | A radiochemical assay which fulfills the required validation criteria has been used for quantification of the in vitro biosynthesis of JH III by the corpora allata of adult females of Blattella germanica throughout the 7 days of the first reproductive cycle. The presence of JH III has been independently confirmed by HPLC and mass spectrometry. Results indicate that rates of JH release increase repidly from day 3 to day 6, which is correlated with oocyte growth. The highest levels of JH release (2.58 ± 1.11 pmol/hr per pair) were obtained from day-6 females. The time course of JH production by CA from day-6 females showed that CA released JH at a linear rate for at least 9 hr. From these results, it can be concluded that titers at high production ages and linearity ranges are satisfactory enough to be used in studies on the regulation of JH production in this species. |
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