The developmental antigens of Nematospiroides dubius |
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| Affiliation: | 1. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;2. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran;3. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;4. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;6. Department of Medical Nanotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;7. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran |
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| Abstract: | The developmental antigen spectrum of Nematospiroides dubius was determined using a combination of metabolic labelling, immunoprecipitation and sodium dodecyl sulphate polyacrylamide gel-electrophoresis (SDS-PAGE) analysis. Antigens produced and assessed in this way were shown to be useful for the discrimination of high (SJL) and low (CBA) responder strains of mice. Interesting differences were also noted between larvae (immunogenic?) and adult (immunomodulatory?) stages, lending support to the hypothesis that adults and larvae may differentially produce immunogenic and immunomodulatory molecules. Experiments were also done to ascertain the optimal conditions for fractionating N. dubius polypeptides, and to produce candidate immunomodulatory molecules in vitro by gene-cloning methodology. |
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