Production of chitinases by Aphanocladium album grown on crystalline and colloidal chitin |
| |
Authors: | Monika Studer Konrad Flück Wolfgang Zimmermann |
| |
Institution: | Institute of Biotechnology, ETH Zürich, 8093 Zürich, Switzerland |
| |
Abstract: | Abstract The deuteromycete Aphanocladium album produced two endochitinases (EC 3.2.1.14) with apparent isoelectric points of 7.1 and 7.6 and seven exochitinases (EC 3.2.1.30) with apparent isoelectric points ranging from 3.8 to 6.4 when grown on a colloidal chitin preparation. With crystalline chitin as carbon source, two endochitinases (p I 7.1 and 7.6) and only one exochitanase (p I 4.9) were detected. The exochitinase p I 4.9, which was produced with both substrates, has a relative molecular mass of 44 000. The different chitinases could be separated by chromatofocusing and their specific activities were determined. |
| |
Keywords: | Aphanocladium album Chitin Chitinase Chromatofocusing |
|
|