Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine |
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| Authors: | Robert Fields and Henry B. F. Dixon |
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| Affiliation: | Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K. |
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| Abstract: | A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is discussed. |
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