Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity |
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Authors: | Katja Witzel Andrea Matros Anders LB Møller Eswarayya Ramireddy Christine Finnie Manuela Peukert Twan Rutten Andreas Herzog Gotthard Kunze Michael Melzer Stephanie Kaspar‐Schoenefeld Thomas Schmülling Birte Svensson Hans‐Peter Mock |
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Institution: | 1. Leibniz Institute of Plant Genetics and Crop Plant Research, Stadt Seeland, Gatersleben, Germany;2. Leibniz Institute of Vegetable and Ornamental Crops, Gro?beeren, Germany;3. Technical University of Denmark, Kongens Lyngby, Denmark;4. Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences, Free University of Berlin, Berlin, Germany;5. Biosystems Engineering, Fraunhofer Institute for Factory Operation and Automation, Magdeburg, Germany |
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Abstract: | Although the physiological consequences of plant growth under saline conditions have been well described, understanding the core mechanisms conferring plant salt adaptation has only started. We target the root plasma membrane proteomes of two barley varieties, cvs. Steptoe and Morex, with contrasting salinity tolerance. In total, 588 plasma membrane proteins were identified by mass spectrometry, of which 182 were either cultivar or salinity stress responsive. Three candidate proteins with increased abundance in the tolerant cv. Morex were involved either in sterol binding (a GTPase‐activating protein for the adenosine diphosphate ribosylation factor ZIGA2], and a membrane steroid binding protein MSBP]) or in phospholipid synthesis (phosphoethanolamine methyltransferase PEAMT]). Overexpression of barley MSBP conferred salinity tolerance to yeast cells, whereas the knock‐out of the heterologous AtMSBP1 increased salt sensitivity in Arabidopsis. Atmsbp1 plants showed a reduced number of lateral roots under salinity, and root‐tip‐specific expression of barley MSBP in Atmsbp1 complemented this phenotype. In barley, an increased abundance of MSBP correlates with reduced root length and lateral root formation as well as increased levels of auxin under salinity being stronger in the tolerant cv. Morex. Hence, we concluded the involvement of MSBP in phytohormone‐directed adaptation of root architecture in response to salinity. |
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Keywords: | barley MSBP plasma membrane proteome analysis root morphology salinity |
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