The intracellular nucleotide‐binding leucine‐rich repeat receptor (SlNRC4a) enhances immune signalling elicited by extracellular perception |
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Authors: | Meirav Leibman‐Markus Lorena Pizarro Silvia Schuster ZJ Daniel Lin Ofir Gershony Maya Bar Gitta Coaker Adi Avni |
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Institution: | 1. School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel;2. Department of Plant Pathology, University of California, Davis, California;3. Department of Plant Pathology and Weed Research ARO, The Volcani Center, Rishon LeZion, Israel;4. School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, IsraelCurrent Address: Donald Danforth Plant Science Center, Saint Louis, Missouri, USA |
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Abstract: | Plant recognition and defence against pathogens employs a two‐tiered perception system. Surface‐localized pattern recognition receptors (PRRs) act to recognize microbial features, whereas intracellular nucleotide‐binding leucine‐rich repeat receptors (NLRs) directly or indirectly recognize pathogen effectors inside host cells. Employing the tomato PRR LeEIX2/EIX model system, we explored the molecular mechanism of signalling pathways. We identified an NLR that can associate with LeEIX2, termed SlNRC4a (NB‐LRR required for hypersensitive response‐associated cell death‐4). Co‐immunoprecipitation demonstrates that SlNRC4a is able to associate with different PRRs. Physiological assays with specific elicitors revealed that SlNRC4a generally alters PRR‐mediated responses. SlNRC4a overexpression enhances defence responses, whereas silencing SlNRC4 reduces plant immunity. Moreover, the coiled‐coil domain of SlNRC4a is able to associate with LeEIX2 and is sufficient to enhance responses upon EIX perception. On the basis of these findings, we propose that SlNRC4a acts as a noncanonical positive regulator of immunity mediated by diverse PRRs. Thus, SlNRC4a could link both intracellular and extracellular immune perceptions. |
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Keywords: | EIX endocytosis immune receptors LeEIX2 NB‐LRR NLR pattern triggered immunity SlNRC4 |
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