The development of sequence-tagged sites (STSs) in Lolium perenne L.: the application of primer sets derived from other genera |
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Authors: | C Taylor K Madsen S Borg M C Møller B Boelt P H Holm |
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Institution: | Department of Plant Biology, The Danish Institute of Agricultural Sciences, Research Center Flakkebjerg, Fors?gsvej 1, DK-4200 Slagelse, Denmark, DK
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Abstract: | Genetic analysis, particularly the development of genetic linkage maps in forage grass species, lags well behind other members
of the Poaceae. Comparative mapping within this family has revealed extensive conservation in gene and marker synteny among chromosomes
of diverse genera. Recently, the ability to transfer mapped STS markers between barley and wheat has been demonstrated. The
transfer of mapped STS markers between cereals and forage grasses could provide PCR-based markers for comparative mapping
in these species providing they amplify homologous sequences. In this study, primers derived from three barley genes of defined
function and a gene from Phalaris coerulescens were used to amplify homologous fragments in Lolium perenne. Primers derived from two barley and two oat cDNA clones were also tested along with eight barley and two Triticum tauchii STS markers. Twenty one primer pairs derived from 18 loci were tested. Eleven primer pairs (52%) amplified homologous sequences
in L. perenne from ten (55%) of the loci targetted. Thirteen new STS markers were generated in L. perenne, of which ten have been mapped in barley or rye and amplify homologous sequences in L. perenne.
Received: 20 October 2000 / Accepted: 13 January 2001 |
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Keywords: | Sequence tagged sites PCR Homology Wheat Barley Lolium Forage grasses |
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