Effect of Staphylococcus aureus serine proteinase on human lymphocyte impairment |
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| Authors: | B Turyna T Ocha?ek |
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| Institution: | Department of Animal Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland. |
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| Abstract: | Assessment of lymphocyte surface membrane integrity by means of trypan exclusion test and longer term survival studies based on 3HTdR uptake and Con A stimulation test were used to estimate effect of Staphylococcus aureus serine proteinase on human lymphocytes in vitro. At concentration of proteinase as high as 10 micrograms/ml, the percentage of trypan-stained lymphocytes increase with enzyme level. In contrast, decrease of 3HTdR level and Con A stimulation is already apparent at concentrations of proteinase ten times lower. Autologous serum exerts strong protective effect. Observed impairment is discussed as significant for local response in inflammation. |
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