Chemiluminescence of Pholasin caused by peroxynitrite |
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| Authors: | Glebska Jolanta Koppenol Willem H |
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| Affiliation: | 1. Jilin Provincial Key Laboratory of Advanced Energy Materials, Department of Chemistry, Northeast Normal University, Changchun 130024, PR China;2. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China;1. School of Chemical Science and Engineering, Shanghai Key Lab of Chemical Assessment and Sustainability, Key Laboratory of Yangtze River Water Environment, Tongji University, Shanghai 200092, China;2. Institute of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China;1. Department of Chemistry and Chemical Engineering, WCSL (World Class Smart Lab) Green Energy Battery Lab., Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea;2. Department of Chemistry and Nanoscience, College of Natural Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea;3. Department of Chemistry and Chemical Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea;1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, Hubei, China;2. School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, Hubei, China |
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| Abstract: | The kinetics of the oxidation of Pholasin by peroxynitrite, which leads to emission of light, were studied. The reaction shows a lag phase, which is smaller at higher peroxynitrite-to-Pholasin ratios. The total light emission approximately doubles from pH 5 to 9 and decreases precipitously to half the pH 5 value at pH 10. Dioxygen and carbon dioxide accelerate the reaction course, but they do not change significantly the reaction yield. Chemiluminescence of Pholasin is suppressed by antioxidants, but no significant shift is noticed in the time at which light emission is maximal. The chemiluminescence intensity is strongly dependent on the potassium concentration, although it is not significantly affected by lithium, cesium, or magnesium; potassium decreases luminescence. The mechanism of the peroxynitrite-induced oxidation of Pholasin may start with the reversible formation of a protein-peroxynitrite intermediate, or a first oxidation product, followed in subsequent steps by decomposition and light emission. However, many questions concerning the mechanism of the light emission remain to be elucidated. |
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