| 烫伤大鼠远隔器官热休克抑制基因表达的研究 |
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| 引用本文： | 郭兴中 刘宇健. 烫伤大鼠远隔器官热休克抑制基因表达的研究[J]. 生理学报, 1997, 49(4): 370-374 |
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| 作者姓名： | 郭兴中 刘宇健 |
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| 作者单位： | 第二军医大学基础部生物化学教研室!上海，200433，第二军医大学病理生理学教研室!上海，200433，第二军医大学遗传学教研室!上海，200433，第二军医大学病理生理学教研室!上海，200433 |
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| 基金项目： | 国家自然科学基金!39400152 |
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| 摘 要： | 为了研究应激是否可诱导完整高等动物远隔器官细胞的热休克反应，在已发现烫伤大鼠肝，脑有热休克蛋白诱导的基础上，本文进一步研究了正常基因表达的抑制。雄性ＳＤ大鼠背部烫伤后，于１０－２４０ｍｉｎ分别断头处死，然后通过斑点印迹法分析热休克抑制基因－１信使核糖核酸的变化。
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| 关 键 词： | 灼伤 应激 热休克反应 基因表达 热休克抑制基因 |
A STUDY ON EXPRESSION OF THE HEAT SHOCK SUPPRESSED GENE IN DISTAL ORGANS OF RATS AFTER SCALDING |
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| GUO XING-ZHONG,LIU YU-JIAN,WANG YI-LUN,XU REN-BAO. A STUDY ON EXPRESSION OF THE HEAT SHOCK SUPPRESSED GENE IN DISTAL ORGANS OF RATS AFTER SCALDING[J]. Acta Physiologica Sinica, 1997, 49(4): 370-374 |
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| Authors: | GUO XING-ZHONG LIU YU-JIAN WANG YI-LUN XU REN-BAO |
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| Abstract: | In order to address the question whether stress in intuct higher animals may inducecellular heat shock response in distal organs, the inhibition of normal gene expressionwas studied on the basis of our previous findings about the induction of heat shock proteins in liver and brain of rats after scalding. Male SD rats were scalded on the back, 10~240 min thereafter decapitated, and the heat shock suppressed gene-1 was quantituted by dot blotting. The results showed that gene-1 decreased rapidly after scalding inboth the organs, and did not recover to control levels even 240 min after scalding. Thedecrease of gene-1 went parallell with the severity of scalding. Thus it may be concluded that stress may induce heat shock response of distant organs in intuct animals. Possible pathological significance of these findings was discussed. |
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| Keywords: | rats burns stress heat shock response gene expression messenger RNA |
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