A simple and rapid method for lithium acetate-mediated transformation of Kluyveromyces marxianus cells |
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Authors: | Antunes Daiane Felberg de Souza Junior Cláudio Galvão de Morais Junior Marcos Antonio |
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Affiliation: | (1) Setor de Biologia Molecular/LIKA and Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil |
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Abstract: | Efficient plasmid transformation of Kluyveromyces marxianus cells of 1.9 × 103 transformant μg−1 DNA with an episomal plasmid was achieved by the use of a simple lithium acetate method with the addition of 10 mM DTT and an increased heat shock temperature of 47 °C. This method is shown to be also efficient for replicative plasmids. Therefore, we suggest its use as a routine method to transform K. marxianus cells. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Dithiothreitol lithium acetate Kluyveromyces marxianus yeast transformation |
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