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Rapid determination of chain length pattern in poly(ADP-ribose) samples |
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| Authors: | Peter Adamietz Reinhard Bredehorst Helmuth Hilz |
| Institution: | Institut für Physiologische Chemie, Universität Hamburg, Germany |
| Abstract: | (3H)poly(ADP-ribose) synthesized from nuclei by incubation with (3H)NAD was released from protein by alkaline treatment and electrophoresed in dodecyl sulfate gels. Individual polymers up to at least 33 units were completely separated according to their chain length. Size distribution was visualized by fluorography of the gels, and quantified by radioactivity determination of sliced gels The method could be applied to crude nuclear extracts. It showed that nuclei of Ehrlich ascites tumor cells produced a poly(ADP-ribose) pattern distinctly different from that of rat liver nuclei. |
| Keywords: | BPB bromophenol blue EAT cells Ehrlich ascites tumor cells ADPR adenosine diphosphate ribose poly(ADPR) homopolymer of adenosine diphosphate ribose with chain length n ≧ 2 |
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