Reduced immunoglobulin G activates complement system with decreased cooperativity |
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Authors: | J.Keith Wright |
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Affiliation: | Department of Biophysical Chemistry, Biozentrum der Universität Basel, CH-4056 Basel, Switzerland |
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Abstract: | Sheep erythrocytes sensitized with intact antibody or reduced and alkylated antibody were lysed by guinea-pig serum indicating that reduced and alkylated antibody bound and activated complement. Reduction of antibody caused erythrocyte lysis to exhibit pseudo-first-order kinetics, while the lysis kinetics of erythrocytes sensitized with intact antibody was sigmoidal. Analysis of erythrocyte lysis by complement according to the von Krogh equation showed that reduction of antibody diminished the von Krogh exponent from 2.8 to 1.3, while the value of remained unchanged at 0.17 (complement dilution). These observations suggested that the sole effect of the reduction of antibody inter-heavy-chain and heavy-light chain disulfide bonds was to diminish the cooperativity of antibody-complement interaction. |
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