Cellular DNA damage by the antitumor protein macromomycin and its relationship to cell growth inhibition |
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| Authors: | Terry A. Beerman |
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| Affiliation: | 1. Department of Experimental Therapeutics Roswell Park Memorial Institute New York State Department of Health Buffalo, New York 14263 USA;2. Grace Cancer Drug Center Roswell Park Memorial Institute New York State Department of Health Buffalo, New York 14263 USA |
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| Abstract: | Macromomycin, an antitumor protein, after purification inhibited HeLa S3 cell growth at low nanomolar concentrations. DNA synthesis was inhibited at drug levels that left RNA and protein synthesis unaffected. Incubation of macromomycin with HeLa S3 cells resulted in a rapid fragmentation of cellular DNA that was detectable at low nanomolar drug levels. Heat denaturation of macromomycin showed that both its ability to inhibit cell growth and to fragment cellular DNA were lost at similar rates. |
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