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Partial amino acid sequence of the preprotein form of the alpha subunit of human choriogonadotropin and identification of the site of subsequent proteolytic cleavage |
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| Authors: | S Birken J Fetherston J Desmond R Canfield I Boime | |
| Institution: | 1. Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, N.Y. 10032 USA;1. Department of Obstetrics & Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110 USA | |
| Abstract: | Messenger RNA isolated from first trimester placentae was translated using radiolabeled amino acids in both the wheat germ and the ascites cell-free systems. The choriogonadotropin α subunit product was purified by immunoprecipitation with a subunit specific antiserum. Its amino acid sequence was partially determined by automated Edman degradation analysis. An NH2-terminal extension of 24 amino acids was found and its partial sequence is:  | |
| Keywords: | α alpha subunit β beta subunit FSH follitropin hCG choriogonadotropin HPLC high pressure liquid chromatography LH lutropin PITC phenylisothiocyanate PTH phenylthiohydantoin RCM reduced and carboxymethylated SDS sodium dodecyl sulphate | |
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