Formation of a hybrid toxin from ricin agglutinin and a non-toxic mutant protein of diphtheria toxin |
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Authors: | Tsuyoshi Uchida Masaru Yamaizumi Yoshio Okada |
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Affiliation: | The Research Institute for Microbial Diseases, Osaka University, Yamada-Kami, Suita, Osaka, Japan |
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Abstract: | CRM45, a non-toxic mutant protein of diphtheria toxin, is treated with glutaraldehyde and conjugated to ricin agglutinin. The hybrid protein thus obtained is purified by gel filtration and affinity chromatography. The toxicity of the purified hybrid toxin is about 8–10 times grater than that of ricin agglutinin when tested in mice and cultured L cells. |
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