溴氰菊酯对神经细胞钙通道和 钙库的激活作用

应用膜片钳全细胞记录方式和显微荧光测钙技术，以MN9D神经细胞为材料研究了溴氰菊酯的作用机理。低浓度(10^-9 mol/L～10^-7 mol/L)溴氰菊酯就能使神经细胞Ca²⁺电流显著增加。10^-9 mol/L，1 min时电流增加平均值为20.64%，5 min时为15.48%，表明溴氰菊酯能激活高电位激活钙通道(L型和N型)，促使Ca²⁺内流，显微荧光测定细胞内自由钙离子浓度（［Ca²⁺］_I）发现，在含Ca²⁺和无Ca²⁺的胞外液中，溴氰菊酯均能使胞内自由钙离子数量增加，表明它能刺激胞内钙库释放Ca²⁺。［Ca²⁺］_I升高对细胞功能影响很大。

Active effect of deltamethrin on calcium channels and calcium store of culture neurons

The effect of pyrethroid insecticide deltamethrin on mesencephalic originated dopaminergic cell line MN9D was investigated by using the patch clamp and Fura 2 microfluorimetric techniques. Whole cell calcium current recordings showed: deltamethrin activated calcium channels (H VOCs) and increased the amplitude of calcium currents greatly by about 20 64% in 1 min and 15 48% in 5 min at very low concentration (10 -9 mol/L). Deltamethrin could increase the Ca 2 ] i in both normal and calcium free bath solutions. This indicates deltamethrin could stimulate the calcium store to release the cytosolic free Ca 2 . We conclude deltamethrin stimulates Ca 2 ] i entry through the calcium channels and release of Ca 2 from intracellular store. Prolonged elevation of Ca 2 ] i will affect many cellular process and cause cytotoxic to the cells.