Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging |
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Authors: | ZhenHua Ren JiaYin Wang ChunLin Zou YunQian Guan Yu Alex Zhang |
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Institution: | Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China. |
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Abstract: | Recently, transplantation of allogeneic and autologous cells has been used for regenerative medicine. A critical issue is
monitoring migration and homing of transplanted cells, as well as engraftment efficiency and functional capability in vivo. Monitoring of superparamagnetic iron oxide (SPIO) particles by magnetic resonance imaging (MRI) has been used in animal
models and clinical settings to track labeled cells. A major limitation of MRI is that the signals do not show biological
characteristics of transplanted cells in vivo. Bone marrow mesenchymal stem cells (MSCs) have been extensively investigated for their various therapeutic properties, and
exhibit the potential to differentiate into cells of diverse lineages. In this study, cynomolgus monkey MSCs (cMSCs) were
labeled with Molday ION Rhodamine-B™ (MIRB), a new SPIO agent, to investigate and characterize the biophysical and MRI properties
of labeled cMSCs in vitro and in vivo. The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging.
Our method is helpful for detection of transplanted stem cells in vivo, which is required for understanding mechanisms of cell therapy. |
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