Anticancer effects of 6-o-palmitoyl-ascorbate combined with a capacitive-resistive electric transfer hyperthermic apparatus as compared with ascorbate in relation to ascorbyl radical generation |
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Authors: | Shinya Kato Ryoko Asada Katsuhiro Kageyama Yasukazu Saitoh Nobuhiko Miwa |
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Affiliation: | (1) Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan;(2) Osaka Butsuryo College, OtoriHigashiMachi 4-410-5, Nishi-ku, Sakai, Osaka 593-8324, Japan;(3) Present address: Radioisotope Centre, Osaka City University Graduate School of Medicine, Asahimachi 1-4-3, Abeno-ku, Osaka 545-8585, Japan;(4) Present address: Laboratory of Bioscience & Biotechnology for Cell Function Control, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan;(5) Present address: Department of Radiological Technology, Faculty of Health Sciences, Butsuryo College of Osaka, OtoriKitaMachi 3-33, Nishi-ku, Sakai, Osaka 593-8328, Japan; |
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Abstract: | The aim of the present study is to determine the anti-proliferative activity of 6-o-palmitoyl-l-ascorbic acid (Asc6Palm) that is a lipophilic derivative of l-ascorbic acid (Asc), on human tongue squamous carcinoma HSC-4 cells by combined use of hyperthermia in comparison to Asc. Asc6Palm or Asc were administered to HSC-4 cells for 1 h, to which hyperthermia at 42 °C was applied for initial 15 min. After further 1–72 h incubation at 37 °C, cell proliferation was determined with Crystal Violet staining. Ascorbyl radical (AscR) in HSC-4 cell suspension was measured by electron spin resonance (ESR), and cell morphology was observed with scanning electron microscopy (SEM). At 37 °C, 4 mM Asc or 0.35 mM Asc6Palm were enough to suppress proliferation of HSC-4 cells. By combined use of hyperthermia at 42 °C, cell proliferation was decreased when compared to 37 °C. After Asc of 4 mM was incubated with HSC-4 cell suspensions at 37 °C or 42 °C for 0–180 min, the signal intensity of ascorbyl radical (AscR) by ESR was not different regardless of the presence or absence of cells at 37 °C, whereas AscR signal was enlarged in the presence of HSC-4 cells at 42 °C. It was suggested that oxidation of Asc occurred rapidly in HSC-4 cells by hyperthermia, and thereby enhanced the anti-proliferative activity. By SEM observation, the surface of HSC-4 cells treated with Asc6Palm revealed distinct morphological changes. Thus, the combined regimen of Asc6Palm and hyperthermia is expected to exert a marked antitumor activity. |
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Keywords: | 6-o-Palmitoyl-l-ascorbic acid Hyperthermia Human tongue squamous carcinoma cells HSC-4 Crystal Violet staining Electron spin resonance (ESR) Scanning electron microscopy (SEM) |
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