Heparin affin regulatory peptide in milk: its involvement in mammary gland homeostasis |
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Authors: | Bernard-Pierrot Isabelle Delbé Jean Heroult Melanie Rosty Christophe Soulié Patrick Barritault Denis Milhiet Pierre-Emmanuel Courty Jose |
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Institution: | Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires FRE CNRS No. 2412, Université Paris Val de Marne Avenue du Général de Gaulle, Créteil, France. |
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Abstract: | HARP (heparin affin regulatory peptide) is a heparin binding growth factor implicated in cellular growth and differentiation. Previously, HARP had been localized in the human mammary, in both alveolar epithelial and myoepithelial cells although HARP mRNAs were only expressed by myoepithelial cells J. Histochem. Cytochem. 45 (1997) 1]. In the present study, we demonstrate that HARP is secreted in human mature milk with concentrations ranging from 17.68+/-6.4ng/ml in mature milk to 59.9+/-11.22ng/ml in colostrum. In vitro, HARP was found to be mitogenic on human mammary epithelial and myoepithelial cell lines and correlated with the expression of its high affinity receptor tyrosine kinase ALK (anaplastic lymphoma kinase). In vivo, ALK is expressed in both mammary epithelial and myoepithelial cells, suggesting that HARP could act in vivo as a paracrine and autocrine growth factor in the regulation of the mammary gland development and its homeostatic maintenance during pregnancy and lactation. |
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Keywords: | HARP Pleiotrophin Human milk Anaplastic lymphoma kinase Mammary epithelial cells |
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