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Marker-free image registration of electron tomography tilt-series |
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| Authors: | Carlos Oscar Sanchez Sorzano Cédric Messaoudi Matthias Eibauer JR Bilbao-Castro R Hegerl S Nickell S Marco JM Carazo |
| Affiliation: | 1. Escuela Politécnica Superior, Universidad San Pablo-CEU, Campus Urb Montepríncipe s/n, E-28668, Boadilla del Monte, Madrid, Spain 2. Biocomputing Unit of the National Center of Biotechnology (CSIC), Madrid, Spain 3. Institut Curie, Centre de Recherche, Orsay, F-91405, France 4. INSERM, U759, Orsay, F-91405, France 5. Max-Planck Institute for Biochemistry, Martinsried, Germany 6. Computer architecture department, Univ de Almería, Almería, Spain |
| Abstract: | BackgroundTilt series are commonly used in electron tomography as a means of collecting three-dimensional information from two-dimensional projections. A common problem encountered is the projection alignment prior to 3D reconstruction. Current alignment techniques usually employ gold particles or image derived markers to correctly align the images. When these markers are not present, correlation between adjacent views is used to align them. However, sequential pairwise correlation is prone to bias and the resulting alignment is not always optimal. |
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