Antifungal Activity of Baicalein Against Candida
krusei Does Not Involve Apoptosis |
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Authors: | Kai Kang Wing-Ping Fong Paul Wai-Kei Tsang |
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Institution: | 1. Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong, China 2. Center of Novel Functional Molecules, The Chinese University of Hong Kong, Hong Kong, China 3. Oral BioSciences, 5/F, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Room 561, Sai Ying Pun, Hong Kong, China
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Abstract: | The present study was designed to evaluate the antifungal activity of baicalein against Candida krusei isolates. Using a broth microdilution assay, baicalein exhibited potent in vitro antifungal activity against C. krusei isolates with a minimum inhibitory concentration of 2.7 μg/ml. Flow cytometric study indicated that baicalein depolarized mitochondrial membrane potential in a concentration-dependent manner. However, mechanistic analyses showed that the intracellular reactive oxygen species (ROS) level was virtually unchanged, and massive DNA fragmentation was not observed in C. krusei isolates after baicalein treatment even at a concentration which was apoptotic in C. albicans. Taken together, we conclude that the antifungal activity of baicalein in C. krusei isolates occurs through perturbation in mitochondrial homeostasis without causing elevation of the intracellular ROS level and does not involve apoptosis. |
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