Lipid-polyethylene glycol interactions: II. Formation of defects in bilayers |
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| Authors: | L T Boni T P Stewart J L Alderfer S W Hui |
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| Institution: | (1) Department of Biophysics, Roswell Park Memorial Institute, 14263 Buffalo, New York |
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| Abstract: | Summary Polyethylene glycol, a known cell fusogen, is found to induce the formation of structural defects in egg phosphatidylcholine multilamellar vesicles, as shown by freeze-fracture microscopy.31P NMR spectra of these vesicles reveal the existence of a nonbilayer (isotropic) phase. The observed disruption in the bilayers is believed to be associated with an intermediate stage of membrane fusion.Abbreviations PEG
Polyethylene glycol
- IMP
Intramembranous particle
- PC
Phosphatidylcholine
- PS
Phosphatidylserine
- SUV
Small unilamellar vesicles
- MLV
Multilamellar vesicles
- DPPC
Dipalmitoyl phosphatidylcholine
- DSC
Differential scanning calorimetry
- DMPC
Dimyristoylphosphatidylcholine
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T
c
Phase transition temperature |
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| Keywords: | Polyethylene glycol bilayer defects multilamellar vesicles 31P NMR freeze fracture X-ray diffraction |
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