Flow-force relationships in mitochondrial oxidative phosphorylation |
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Authors: | H Woelders J Putters K van Dam |
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Affiliation: | Laboratory of Biochemistry, University of Amsterdam, PO Box 20151, 1000 HD Amsterdam, The Netherlands |
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Abstract: | The rates of oxidation and phosphorylation in isolated rat-liver mitochondria have a steep dependence on the protonmotive force (delta mu H+) across the membrane. These experimentally observed relationships proved to be independent of the way in which delta mu H+ was varied. These results were obtained when the membrane potential (delta psi) was calculated from the distribution of K+ (in the presence of valinomycin). When triphenylmethylphosphonium (TPMP+) was used as a probe for delta psi, slightly different flow-force relationships were obtained. We conclude that unique relationships exist between delta mu H+ and the rates of oxidation and phosphorylation, and that under some conditions the behaviour of the probe TPMP+ is anomalous. |
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